GATK caveat

1. 选择/过滤

VariantFiltration: Filter variant calls based on INFO and/or FORMAT annotations
output: A filtered VCF in which passing variants are annotated as PASS and failing variants are annotated with the name(s) of the filter(s) they failed.
SelectVariants:    Select a subset of variants from a VCF file.
output:
1.如果一个值缺失,VariantFiltration会认为这条值所在的记录通过检查,而SelectVariants认为这条记录不能通过检查。

2.foobar

 

notes of ANNOVAR

1. 坐标系: By default, 1-based coordinate system is used.

2. 核心程序: annotate_variation.pl

3. 注释类型: gene-based (-geneanno), region-based (-regionanno) and filter-based (-filter) annotations.

4. 输出结果:

a. The first file contains annotation for all variants, by adding two columns to the beginning of each input line.

b. The second output file contains the amino acid changes as a result of the exonic variant.

5. 重点定位:

What about GFF3 file for new species?(http://annovar.openbioinformatics.org/en/latest/user-guide/gene/)

gff3ToGenePred                                                                               (http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86_64/)

星露谷物语 玩法总结

首先,我们聊一聊,星露谷物语里面,一天可以怎么度过。

第一,你可以选择留在你的农场,你有很多事情可以做。去抚摸你的家畜、宠物;去搜集农产品;去整理生产设备;去清点物资;去布置房子;去对农作物进行再加工和生产,然后出售;去和你的妻子和孩子互动。

第二,你可以选择去和村民互动,拜访他们,给他们送礼;

第三,你可以选择去挖矿,砍树,搜集物资;

第四,你可以选择去钓鱼;在不同时间,不同的地点,可以钓上来不同的鱼。

第五,你可以选择去打怪。坐上潘姆开的车去沙漠,然后去沙漠山洞打怪,搜集物资。带上炸弹,才能够下的足够深。