Key points of the analysis of microarray – 基因芯片分析要点

  1. biological replicates – 生物学重复
    Five or more is usually robust for micro-array studies
    五个及以上的样本数对基因芯片研究来说才是具有鲁棒性的。
  2. qPCR validation
    Micro-array may give many false positives so it is usually necessary to validate the differential expression observed in some of the key genes.
    基因芯片可能产生许多的假阳性结果,所以验证部分关键的差异表达基因通常来说是必须的。